This dynamic rendition of the song comes from the band’s appearance at Japan’s Summer Sonic Festival.
Queen’s Brian May said, “Like Live Aid in 1985, we believe this concert will be a milestone."
Also headlining are Pharrell Williams, Alicia Keys, OneRepublic, H.E.R. and Carole King.
The band will on the road in the US throughout July and August with their ‘Rhapsody’ tour dates concluding on the 23 August at the Spectrum Center in Charlotte, North Carolina.
The original version of the song is piano-based and features markedly different lyrics.
The Queen frontman performed ‘Bohemian Rhapsody’ during his own audition for the show in 2009.
The politically-charged new song is the star's first solo work in more than five years.
'A Star Is Born' now has the most weeks at No. 1 for a live-action film soundtrack since Disney Channel’s TV movie 'High School Musical 2' in 2007.
The film will follow the behind-the-scenes activities between Lambert and original Queen members Brian May and Roger Taylor since Lambert’s American Idol debut in 2009.
Last year's event saw headlining sets from Queen + Adam Lambert, The Killers and Arctic Monkeys.
'Bohemian Rhapsody', the widely-acclaimed biopic about late Queen frontman Freddie Mercury, is up for five Oscars including Best Picture.
The film soundtrack is already becoming one of the band's fastest-selling albums throughout the world.
The epic 23-date arena and stadium tour is set to run from mid-July through late August with an all new concert experience.
Queen + Adam Lambert have announced 'The Crown Jewels': a limited 10-date run of shows at the Park Theater at Park MGM in Las Vegas for September.
Queen + Adam Lambert have been confirmed to headline Scotland's TRNSMT Festival with Liam Gallagher, Stereophonics and The Killers
Following their rapturously-received UK shows over the winter, Queen + Adam Lambert have announced further UK and European shows for 2018